Potato Dextrose Agar

Potato Dextrose Agar (PDA) is a common microbiological growth media made from potato infusion and dextrose. Potato Dextrose Agar is the most widely used method for growing fungi and bacteria in laboratory environments. It is usually made of 1000 ml of water, 4g of sliced potatoes, 20g of dextrose, 20g agar powder, under a temperature of 25°C. Potato Dextrose Agar is used to cultivate, isolate, and enumerate yeasts and molds from food and other materials. It is a medium that complies with the health requirements recommended by the American Public Health Association. Potato and carbohydrate infusion can promote the growth of yeasts and molds while the low pH percentage increases the growth of other bacterial flora.

Potato Dextrose Agar (PDA) is used to cultivate fungi. PDA is a medium for yeasts and molds that are supplemented with acid or antibiotics in order to foster bacterial growth. It is most commonly used for plate count methods in foods, dairy products, and testing cosmetics. The purpose of this method is to grow yeasts and molds within the clinical laboratory setting. The potato base infusion is so nutritionally rich that it encourages mold sporulation and pigment production. It is composed of dehydrated potato infusion and dextrose that increase fungal growth, where agar acts as the solidifying agent. Many of the standard procedures associated with this medium use sterile tartaric acid to lower the pH levels, which makes for an inhibited bacterial growth. Meanwhile, chloramphenicol acts as the selective agent that guarantees bacterial growth between competing microorganisms from mixed specimens, while simultaneously allowing the selective isolation of fungi.

Potato Dextrose Agar is used to detect yeasts and molds in dairy products and prepared foods. It can also be used to cultivate yields and molds from clinical specimens. It is recommended to use tartaric acid when examining microbes in said experiments. PDA is used to prepare and maintain fungal test strains used in growth tests. It is not intended to be used to diagnose diseases or other conditions in humans. It is most commonly used to cultivate fungi, especially in the preparation of the aspergillus brasiliensis test strain The potato and dextrose extract provide a nutritional testing base that produces mold sporulation and pigment generation. Meanwhile, chlortetracycline is recommended to use when enumerating microbes. Additionally, chloramphenicol is recommended when cultivating fungi from mixed samples.

Mycologists use PDA as a medium for yeasts and molds that can be identified according to their morphological characteristics and their pigmentation in culture. Therefore, PDA serves the purpose of cultivating and separating pathogenic and non-pathogenic fungi from one another. It is made of potato infusion and dextrose extract, these materials support the growth of bacteria and fungi. The media may be supplied with certain acids or antibiotics in order to grow bacteria that can get in the way of yeasts and molds, while agar acts as the solidifying agent. PDA is normally used in the dairy industry to detect yeasts and molds in product samples with the help of tartaric acid. When enumerating yeasts and molds in cosmetics, PDA is supplemented with chloramphenicol. It is also used to isolate and enumerate yeasts in clinical samples. Finally, it is employed to simulate sporulation.

To prepare potato infusion, the first step is to take 200gm of potato for 1L of PDA media preparation. The next steps are to wash the potato to remove the dirt, then peeling off the skin and dicing them, then adding the pieces to 1L of distilled water, then boiling the water for about 25 min on a hot plate, and finally collecting the extract through the muslin cloth. To avoid the preparation process though, infused forms of potato are being replaced with potato starch-extract powder at a wide commercial level. Some of the indispensable instruments to make this formula are glass breakers, conical flasks, spatulas, measuring cylinders, pH meters, weighing balances, distilled water, butter paper, magnetic stirrer and pellet, pipettes and tips, petri plates and test tubes, as well as hot plates.

TLS offers Potato Dextrose Agars (PDA) with 500g per bottle. The agar is used to isolate, identify, and maintain yeasts and molds from dairy and food products. PDA Agar is suitable for the detection and enumeration of heat-resistant molds in thermally processed fruits and fruit products, and preparing medium for Aspergillus niger. The principle is that the potato flour leaching contributes to various types of mold growth. Meanwhile, glucose provides energy, the agar acts as a medium coagulant, and chloramphenicol inhibits the growth of bacteria. The instructions or use are to dissolve 40g of potato agar in 1 L of distilled water, and then autoclave 15 minutes at 121°C. Storage conditions indicate that the container must be kept tightly closed, stored in a cool, dry place, away from bright light, for a period of 3 years. The formulation consists of 200g of potato infusion, 20g of glucose, and 15g of agar.