Buffered Peptone Water (500g/Bottle)

Buffered Peptone Water (500g/Bottle)

  • Use: Pre-enrichment of Salmonella spp.
  • Characteristics: Pre-enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity
  • Principle: 
  • Peptone provides carbon and nitrogen sources to meet the needs of bacterial growth.
  • Sodium chloride maintains osmotic equilibrium.
  • Potassium dihydrogen phosphate and disodium hydrogen phosphate act as buffers.
  • Instructions: 

Suspend 20 g in 1 liter of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121 °C for 15 minutes. Pour into sterile petri plates. Dry the surface of the gel before inoculation.

  • Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Formulation per liter:

Tryptone

10.0 g

Sodium chloride

5.0 g

Disodium hydrogen phosphate

3.5 g

Potassium dihydrogen phosphate

1.5 g

Final pH

7.0 ± 0.2



Quality control:

Item

The name of strain

PR/G

Reaction

1

Salmonella typhi

Good

Cloudy broth

2

Salmonella typhimurium 

Good

Cloudy broth

3

Paratyphoid Salmonella

Good

Cloudy broth

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